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After the 48 h, the supernatant was removed and a 25 mM of culture medium containing 0.5 mg/mL of MTT salt solution was added to each well and it was again adjusted in a 5% carbon dioxide incubator at 37 C for 4 h

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The instant she saw the letter she squinted her eyes and bent her lips in a tough tiny smile that advanced her age immeasurably

We should consider George was 35 when he met Toni at 43, both in legal terms were single status in 1949