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That mix is one reason DSIP sits in a gray zone

After 15-20 punctures, the stopper may not reseal properly, compromising the sterile environment

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Each reaction mixture was prepared using 10 l QuantiFast SYBR Green PCR master mix, 4 l of cDNA template with 1 M of each primer in a total reaction volume of 20 l

Some reports involved other peptides or therapies at the same time, and the available information did not allow FDA to prove that BPC-157 caused the events