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Key advantages: Oral administration (no injection burden) Scalable manufacturing Potential for combination therapies Broader patient adherence However, these molecules also reintroduce important questions: Will small-molecule receptor activation alter tissue selectivity

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However, the response becomes maladaptive with persistent volume and pressure overload 3

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Islets were again washed three times with PBS, then incubated in permeabilizing blocking buffer for 1 h at room temperature before incubation with primary antibody overnight at 4 C