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The collected cell lysate was vortexed for 5 s and centrifuged at 12,000 rpm for 5 min at 4 C
Other copper peptides studied include DAHK (the N-terminal copper-binding domain of human serum albumin), which has high copper affinity but primarily serves a copper transport rather than signaling function
Insulin, another large peptide administered during pregnancy and lactation for decades, transfers minimally into breast milk despite systemic use
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