The fixed cells were treated with 20 g/mL RNase A and 10 g/mL propidium iodide in PBS at 37 C for 30 min
Also, our results were confirmed by previous findings that reported that MIC of vitamin C against gram-negative bacteria ranged between 2- 16 mg/mL [14, 20, 21]
Our work is supported by National Natural Science Foundation of China (NSFC) #82205069 awarded to CZ
Both the electrical potential difference and the proton concentration gradient that are set up provide the energy used to generate ATP as the protons are drawn back, thermodynamically downhill, into the mitochondrial matrix through the ATP synthase complex (Figure 1)
A Shimadzu LC20AD, Ultra Performance Liquid Chromatography (UPLC) system (Shimadzu Corporation, Kyoto, Japan) equipped with Shimadzu 8040 triple quadrupole (QqQ) mass analyzer (Shimadzu Corporation, Kyoto, Japan) with an electrospray ionization source (ESI) operated in the positive charge mode for the quantification of 1 and 2
Most clinical studies show no significant increase in blood glutathione levels from standard oral supplementation
