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2011 Jul

Substrates of NAT1 include aminobenzoic acid, aminosalicylic acid, the bacteriostatic antibiotics sulfamethoxazole and sulfanilamide, 2aminofluorene and caffeine (Ginsberg et al., 2009)

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Integrating ChIP-seq, HiC, and PRO-seq datasets, we revealed broad functional binding of NRF2 at enhancer elements, including NRF2-dependent enhancer-derived RNA (eRNA) synthesis and extensive linkages between NRF2 target promoters and enhancers

Briefly, once proteins have been spotted, the membrane strips were dried and then non-specific sites were blocked by soaking in 5% BSA in TBS-T (20 mM Tris-HCl, 150 mM NaCl, 0.05% Tween20) for 30 minutes