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The slides were subsequently rinsed with TBS plus Tween 20, treated for 30 min at room temperature with N-Histofine mouse MAX PO (Nichirei, Tokyo, Japan), rinsed with TBS plus Tween 20, incubated in diaminobenzidine solution containing 0.01% hydrogen peroxide for the peroxidase coloring reaction, and counterstained with Mayer's hematoxylin
Similar to Rac1, the increased Cdc42 activity was colocalized with actin filaments and the inhibition of Cdc42 resulted in decreased actin filaments, supporting the involvement of actin changes in AD pathogenesis (Mendoza-Naranjo et al., 2007)