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Calcium supplements (NPES, PES, CaCO3) were administrated at 2.5% concentration mixed into chow feed (15g/days)

doi: 10.1016/j.intimp.2024.112010 154 SunJZhangYWangCRuanQ

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This key conversion is necessary to provide l-methylfolate for the one-carbon transfer reactions (methyl donations) needed for purine/pyrimidine synthesis during DNA and RNA assembly, for DNA methylation, and to regulate homocysteine metabolism (Figure 2)