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The attempts to develop new antibiotics have been costly and inefficient, as many potential drugs entering clinical trials (9099%) have been unsuccessful [25, 51]

After centrifugation at 15,000 g for 50 min, the cell supernatant was incubated with GST beads (Cytiva, Glutathione Sepharose 4B, 17075605) at 4 C for 1.52 h

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It is primarily sold as a research chemical and is associated with safety risks, including inconsistent product quality and potential contamination due to lack of regulation