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Extended Half-Life and Sustained Receptor Engagement The 13-amino acid N-terminal extension and Arg3 substitution reduce IGFBP binding by 7080%, extending the effective half-life from approximately 10 minutes to 2030 hours in rodent plasma resulting in prolonged IGF-1R activation in cell culture and animal models that is not achievable with native IGF-1 or shorter analogues

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NADPH levels were assessed by measuring the fluorescence ratio at 407 and 482 nm (Olympus IX83 microscope), with quantification performed in ImageJ, as described previously 29