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These proteases include cell surface transmembrane protease/serine (TMPRSS) proteases, cathepsins , furin, elastase, factor Xa, and trypsin 70

RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

Three possibilities might explain this phenomenon

The levels of Fe 2+ in each of tissue sample were calculated based on this standard curve

Thus, the factor, S , multiplying the V max values of GCL and GPX, will be S = 1 for males and S = 1.8 for females