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Western blot Cells were harvested and proteins were extracted using cell lysis buffer supplemented with 0.3% phenylmethylsulfonyl fluoride and proteinase and phosphatase inhibitors

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Considering the heterogeneity among ME/CFS patients, however, it is unlikely that one treatment will suit all ME/CFS patients

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Furthermore, the researchers observed a significant colocalization of pCREB with doublecortin (DCX), a marker of neuronal precursors, suggesting that CREB activation might be closely linked with the neurogenic process, specifically affecting neuronal rather than glial cells