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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)

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L.et al (2004a)

In general, glycemic control is an accepted concept in TBI management [27, 38], and supplementation with additional substrates such as lactate (intravenously or via CMD) may also show some clinical utility in reducing complications, like intracranial hypertension [44], when metabolic targets are better defined

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