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The qRT-PCR protocol included preincubation at 95 C for 30 s for 1 cycle
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Nat Neurosci 9:119126 Shashidharan P, Huntley GW, Murray JM, Buku A, Moran T, Walsh MJ, Morrison JH, Plaitakis A (1997) Immunohistochemical localization of the neuron-specific glutamate transporter EAAC1 (EAAT3) in rat brain and spinal cord revealed by a novel monoclonal antibody
Neurological symptoms such as tingling or numbness are more commonly associated with B12 deficiency rather than excess and warrant medical assessment
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