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6E, Supplementary Fig
The full-length, codon-optimized genes encoding human mTOR, RAPTOR and MLST8 were cloned into pCAG vectors (mTOR with TEV-cleavable 2SF, RAPTOR with uncleavable 2SF and MLST8 with uncleavable 2SF)
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Determination of kidney function markers Serum creatinine, urea, and blood urea nitrogen (BUN) levels were determined using detection kits of Spectrum Diagnostics, Cairo, Egypt
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The cryo-EM data show inhibitor 9 bound in the doorstop pocket, indicating that the design strategy, based on the initial fragments found in X-ray co-crystal structures, is successful
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