CCK8 cytotoxicity assay Cells were plated into 96-well plates (2 10 3 cells/100L/well) and cultured in growth medium overnight
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Additionally, ferroptosis-derived ROS can activate the AMPK/ULK1 pathway to initiate autophagy (67), although the precise molecular cascade in LC cells is not fully elucidated
Genes encoding WRKY transcription factors, PR-proteins, a phenylalanine ammonia-lyase (PAL) and a GST were up-regulated in infected berries (Agudelo-Romero et al., Vitis ) species may resist fungal infections by accumulating secondary metabolites like stilbenoid phytoalexins (trans-resveratrols)
Body weight, body length, tibia length, epiphyseal plate width, and serum levels of insulin-like growth factor (IGF)-I were measured after oral administration of 4 mg/kg of MK-677 for 6 weeks to investigate growth-promoting effects
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