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Figures shown are based on internal clinical data and do not constitute a promise or guarantee of specific results

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The nuclear pellet was washed 4 times with the hypotonic lysis buffer after centrifugation at 1500 g for 3 min and then lysed in Reporter lysis buffer (E3971, Promega, USA), followed by incubation on ice for 10 min with a 21 G needle (60016589, KND, Shanghai, China), and finally centrifuged at 17,000 g for 5 min at 4 C