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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

The data discussed here summarize some of the roles of endogenous estrogens, estrogen-based therapies, and estrogen-like molecules on brain homeostasis, particularly in neurogenesis, gliogenesis, and regulation of the neuroprotection/neurodegeneration balance

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This connection between MOTS-C and lifespan has fueled interest in the peptide as an anti-aging compound with metabolic benefits that extend well beyond simple weight management