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Certain groupssuch as older adults, vegetarians, individuals with gastrointestinal disorders, and those taking specific medications (e.g., metformin or proton pump inhibitors)may develop a B12 deficiency

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Glucagon activation increases thermogenesis (heat production), enhances fat oxidation, and elevates metabolic rate

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A vegetarian diet which contains no animal products (including milk products or eggs) does not supply any Vitamin B12

Studies indicate that long-term metformin use can reduce serum B12 blood test levels by up to 20%, necessitating regular monitoring and preventive B12 supplementation protocols