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Optimization of sample preparation conditions To ensure efficient cell lysis, protein precipitation, and simultaneous stabilization of oxidation-prone thiols at a low pH, three common acidic extraction solutions were evaluated for deproteinization efficiency (Fig

Specifically, DON-15-GlcA accounts for 58.2% 8.7% of excreted DON, while unmodified DON and DON-3-glucuronide (DON-3-GlcA) account for 27.4 11.8% and 14.4 6.7%, respectively (Vidal et al