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DOI: Received: Accepted: Published: Publisher Name: Springer, Cham Print ISBN: 978-3-030-81304-8 Online ISBN: 978-3-030-81304-8 eBook Packages: Living Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

Calibration standards were prepared by spiking 50 L of human plasma with appropriate amount of thiols to obtain the following concentrations: 20, 40, 60, 100, 200, 300, 600 nmol/mL plasma for Cys, 2, 4, 6, 10, 20, 30, 60 nmol/mL for Hcy, GSH and 0.1, 0.5, 1.0, 2.0, 4.0, 5.0, 10.0 nmol/mL for N-Hcy-Lys

Reducing agents such as dithiothreitol (DTT) and reduced glutathione are commonly used in cell-free reactions to prevent unwanted oxidation

269 Other Cys residues in HDACs are sensitive to H 2 O 2

LCLT is the form used in most exercise performance and recovery clinical trials (including research on reducing exercise-induced muscle damage)

These mice survived for a total of 25 days and naturally died, which we defined as the survival endpoint as illustrated in Fig