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Graphical Abstract Similar content being viewed by others Abbreviations DLAT : Dihydrolipoamide S-acetyltransferase FDX1: Ferredoxin 1 GCLC: Glutamate-cysteine ligase catalytic subunit ICB: Immune checkpoint blockade ICD: Immunogenic cell death L-BSO: L-Buthionine-(S,R)-sulfoximine LIAS: Lipoic acid synthetase SLC31A1: Solute carrier family 31 TIME: Tumor immune microenvironment TNBC: Triple-negative breast cancer Funding This work was supported by the National Key Clinical Specialty Construction Program, the Science and Technology Program of Chengdu (2024-YF05-00487-SN), the Science and Technology Program of Tibet Autonomous Region (XZ202401RK0005), the Sichuan Science and Technology program (2023YFS0133), the Natural Science Foundation of Xiamen Municipality (3502Z202374035)

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Madewell ZJ, Keita AM, Das PM, Mehta A, Akelo V, Oluoch OB, Omore R, Onyango D, Sagam CK, Cain CJ, Chukwuegbo C, Kaluma E, Luke R, Ogbuanu IU, Bassat Q, Kincardett M, Mandomando I, Rakislova N, Varo R, Xerinda EG, Dangor Z, du Toit J, Lala SG, Madhi SA, Mahtab S, Breines MR, Degefa K, Heluf H, Madrid L, Scott JAG, Sow SO, Tapia MD, El Arifeen S, Gurley ES, Hossain MZ, Islam KM, Rahman A, Mutevedzi PC, Whitney CG, Blau DM, Suchdev PS, Kotloff KL

This three-pronged approach creates a powerful, synergistic effect

This is supported by observations that blood homocysteine decreases in individuals undergoing male to female transition, and vice versa in those undergoing female to male transition (22)

Lyophilized (freeze-dried) powder: Room temperature storage: stable for weeks to months depending on peptide Refrigerator storage (2-8 C): stable for months to over a year Freezer storage (-20 C): stable for years in some cases Keep sealed, dry, and away from light Moisture is the primary enemy of lyophilized peptides Reconstituted solution: Must be refrigerated at 2-8 C at all times Typical stability: 4-6 weeks for most peptides Never freeze reconstituted solutions Use bacteriostatic water (not sterile water) for multi-use vials Each needle puncture introduces contamination risk The difference in stability is dramatic